Made Scientific
Gene therapy viral vector manufacturing in CDMO facility

Gene Therapy Vector Manufacturing

GMP viral vector manufacturing for gene therapy programs — AAV, lentiviral, and retroviral platforms. From process development and characterization through clinical and commercial-scale vector production.

Gene therapy is entering a commercial inflection point — 245 companies, 87 clinical stage, 7 approved products, 150+ active trials. Vector manufacturing capacity is the critical constraint. Made Scientific is investing in the vector manufacturing capacity and analytical capabilities that gene therapy developers need now.

  • • Process development (upstream & downstream)
  • • Cell line development & banking
  • • GMP viral vector production (AAV & lentiviral)
  • • Analytical & characterization (titer, potency, capsid ratio)
  • • Scale-up & tech transfer
  • • Regulatory support (IND through BLA)
Contact us to discuss your vector program

Our Capabilities

From construct optimization through commercial-scale GMP production — every step needed to bring your gene therapy vector from bench to patient.

Process Development

Process Development

Upstream and downstream optimization — transfection, harvest, purification, and formulation — to maximize vector yield, purity, and potency for your specific construct.

  • • Transfection and harvest optimization
  • • Purification and formulation development
  • • Yield and potency maximization
  • • Construct-specific protocol design
Cell Line Development

Cell Line Development

Producer and packaging cell line development and banking — including suspension-adapted HEK293 lines for scalable AAV and lentiviral production.

  • • Producer and packaging cell lines
  • • Suspension-adapted HEK293 systems
  • • Cell bank creation and characterization
  • • Stability and passage qualification
GMP Manufacturing

GMP Manufacturing

GMP viral vector production in purpose-built suites — from bench-scale to 200L+ bioreactors with closed-system processing and full environmental monitoring.

  • • Adherent and suspension production systems
  • • Bench-scale through 200L+ bioreactors
  • • Closed-system processing workflows
  • • Environmental monitoring and controls
Analytical & Characterization

Analytical & Characterization

Full vector characterization: titer (genome copies), potency (transduction, expression), identity (sequencing), purity (HCP, DNA), capsid ratio (AUC, CDMS), and safety (RCL/RCR).

  • • Titer, potency, and identity testing
  • • Purity analysis (HCP, residual DNA)
  • • Full/empty capsid ratio (AUC, CDMS)
  • • RCL/RCR safety testing
Scale-Up & Tech Transfer

Scale-Up & Tech Transfer

Technology transfer from your development lab — including process comparability, scale-up studies, and engineering batch production.

  • • Technology transfer execution
  • • Process comparability studies
  • • Engineering batch production
  • • Scale-up validation and optimization
Regulatory Support

Regulatory Support

IND-enabling and BLA-supporting manufacturing — including CMC documentation, comparability protocols, potency strategy, and regulatory filing support.

  • • CMC documentation and strategy
  • • Comparability protocol development
  • • IND and BLA filing support
  • • Health authority interaction preparation

Two Vector Platforms, One Manufacturing Partner

Both major gene therapy vector classes — AAV for in vivo delivery and lentiviral for ex vivo — with the process development depth to optimize for your specific program.

AAV Vectors

In vivo gene delivery

  • Multiple serotypes (AAV1, 2, 5, 6, 8, 9, rh10, and novel capsids)
  • Triple transfection and baculovirus/Sf9 production systems
  • Full/empty capsid enrichment (AUC, CsCl, chromatography)
  • Adherent and suspension production (up to 2000L)
  • Potency assay development (in vitro transduction, qPCR, ddPCR)

Lentiviral Vectors

Ex vivo gene delivery

  • 3rd and 4th generation lentiviral systems (self-inactivating)
  • Transient transfection and stable producer cell line development
  • High-titer production for CAR-T and gene therapy applications
  • RCL (replication-competent lentivirus) testing and clearance
  • Integration site analysis and biosafety characterization

Our Facilities

Our GMP facilities are designed specifically for cell and gene therapy and actively support concurrent programs across development stages.

Commercial Manufacture & Development Center of Excellence

Made Scientific Headquarters

Located at the heart of innovation in Princeton, New Jersey, our 60,000 ft² facility is equipped to take your therapy from early development to GMP manufacture.

Manufacture & Hybrid Client-in-Plant Operations

Made Scientific Clinical Production

Strategically designed for flexibility, our Newark site offers 15,000 ft² of GMP-ready space to support client-dedicated and hybrid operational models.

Made Scientific Foundry: Workforce Development & Training Center

Workforce Development & Training

We're investing in the future of cell therapy through both hands-on and digitally accessible GMP-inspired training. Located on the NJIT campus, our center has supported over 275 trainees across industry and academia.

Made is easy to work with. We've got your back.

Flexible engagement models for gene therapy programs at every stage.

Traditional & Dedicated Service

All Clients

Full-service vector manufacturing in Made's GMP suites. From upstream production through purification and release — your team maintains oversight.

Hybrid Client-In-Plant Operations

Mid-to-Late Stage Clients

Your scientists work alongside ours. Critical for complex vector programs where deep construct knowledge must transfer with the manufacturing process.

Custom Build-outs & Partnerships

Late-Stage and Commercial Clients

Dedicated vector suites configured for your platform — from upstream bioreactor systems to downstream purification and analytical infrastructure.

Why Made Scientific for Gene Therapy

Deep vector biology expertise

Our scientists understand vector biology — not just manufacturing processes. From capsid engineering to tropism optimization, we bring scientific depth that accelerates process development.

Analytical capabilities

Gene therapy demands the most sophisticated analytics in biologics. Our team develops the assays that characterize your vector completely — not just for release, but for process understanding.

Manufacturing capacity

Purpose-built GMP suites with bioreactor capacity up to 2000L. When your program succeeds, we have the capacity to manufacture at commercial scale — not a waitlist.

Multi-product capability

Segregated manufacturing suites support multiple vector programs simultaneously. Changeover and cleaning validation protocols ensure product safety across different vector types.

Ready to Solve Your Vector Supply Challenge?

Have a question for our sales team? Contact us to explore how our expertise and capabilities can help you achieve your goals.

Contact Us
Scientist in Made Scientific cleanroom